We hypothesized that IMQ-induced dermatitis in mice can serve as a model for the analysis of pathogenic mechanisms in psoriasis-like dermatitis and assessed its IL-23/IL-17 axis dependency. This rapid and convenient model allows further elucidation of pathogenic mechanisms and evaluation of new therapies in psoriasis. We hypothesized that IMQ-induced dermatitis in mice can serve as a model for the analysis of pathogenic mechanisms in psoriasis-like dermatitis and assessed its IL-23/IL-17 axis dependency. This rapid and convenient model allows further elucidation of pathogenic mechanisms and evaluation of new therapies in psoriasis. This prompted us to assess whether IMQ-induced skin inflammation in mice provides a bona fide model representing most features of human psoriasis, thereby focusing on the involvement of the IL-23/IL-17 axis. IMQ-Induced psoriasis is a useful mouse model for evaluation of new therapies for psoriasis and dermatitis. Washington Biotechnology is pleased to offer this model.
Also back hair should be removed completely by Veet cream/other depilatory cream to see a good effect of IMQ. We hypothesized that IMQ-induced dermatitis in mice can serve as a model for the analysis of pathogenic mechanisms in psoriasis-like dermatitis and assessed its IL-23/IL-17 axis dependency. This rapid and convenient model allows further elucidation of pathogenic mechanisms and evaluation of new therapies in psoriasis. The results of this particular whole blood assay demonstrate a novel technique useful in elucidating patient cohorts presenting with autoinflammatory pathophysiologies. Imiquimod-induced dermatitis in mice closely resembles human psoriasis. It is therefore a good model for the evaluation of new therapies for psoriasis. Clobetasol antagonizes the IMQ-induced thickening of the ear, while tacrolimus only has a slight effect.
PLOS ONE promises fair, rigorous peer review, broad scope, and wide readership a perfect fit for your research every time. As IL-6 is important in the pathogenesis of psoriasis, anti-IL-6 has been discussed as a new treatment option of psoriasis in humans 15, but stays disputed. In the model of IMQ-induced psoriasis-like skin disease, IL-6 is important for recruitment of neutrophils 24. The treatment of these mice with an anti-IL-6 antibody led to a reduction of neutrophil micro-abscesses in the skin 27, showing the importance of IL-6 in the context of myelomonocytic cells. Fat-1 IMQ-induced mice exhibited significantly lower levels of inflammatory cell-like T helper 17 cells (Th17 cells) and higher levels of regulatory T cells (Treg cells) in the spleen as compared with the WT IMQ-induced mice. In the present study, we used this fat-1 transgenic psoriasis mouse model to establish n-3 PUFAs as a therapeutic agent for psoriasis and to examine the molecular mechanisms underlying this effect. 15 min at 4C, then evaluated for the levels of inflammatory factors. At the beginning of the treatment, the WT and fat-1 mice were weight matched using a CS 200 balance (Ohaus, Pine Brook, NJ, USA). These results suggest that PF inhibits IMQ-induced psoriasis by regulating Th17 cell response and cytokine secretion via phosphorylation of Stat3. IMQ is used in the topical treatment of genital and perianal warts caused by the human papilloma virus (Beutner and Tyring, 1997) and actinic keratoses and superficial basal cell carcinomas (Fanti et al. These effects are manifested as symptoms of dermatitis that closely resemble human psoriasis, and this process is reported to be dependent on the IL-23/IL-17 axis (van der Fits et al. (2015)), IMQ-induced skin inflammation in mice represents a useful model of human psoriasis.
RESULTS: Imiquimod treatment induced a psoriasis-like skin inflammation. We found that Clock mutation ameliorated IMQ-induced dermatitis, whereas the Per2 mutation exaggerated IMQ-induced dermatitis, when compared with wild-type mice associated with decreased or increased IL-23 receptor (IL-23R) expression in?/?(+) T cells, respectively. The clinical improvements were evaluated by a dermatologist. In this context, we developed a new acute Ps and PsA-like disease model that appears after exposure to Saccharomyces cerevisiae mannan in certain mouse strains. Here, we have investigated skin inflammation in a mouse model where the kindlin-3 binding site in the beta2-integrin has been mutated (TTT/AAA-beta2-integrin knock-in), leading to expressed but dysfunctional integrins. Curbing Inflammation in Skin Wound Healing: A Review. Here, we have exposed the skin of mice to imiquimod (IMQ), which induces IL-23 dependent psoriasis-like inflammation7,8. MD Biosciences news relating to new product releases and new preclinical service offerings. Insights for Preclinical Drug Discovery and Development Challenges. Primary neurons from mice are a good model system to examine the structure and function of neurons and have been widely used very successfully by the neuroscience community. With the earlier launch of the IMQ-induced model and now the IL-23 psoriasis model, MD Biosciences offers a range of rapid models for dermal conditions such as dermatitis and psoriasis.